Professor Douglas D Frey

Education

  • Ph D, Chemical Engineering — UC Berkeley (1984)
    Foam Spray Drying of Foods

Publications

  • Development of a novel analytical sensor utilizing Ni-NTA and His-tag affinity for at-line measurement of binding proteins 2024 IFPAC Annual Meeting 2024 Revati Kadolkar, Vikash Kumar, Venkatesh Srinivasan, Sanjeev Kumar, Douglas D Frey, Govind Rao
  • Purification challenges for the portable, on-demand, point-of-care production of biologics 2022 no · Current Opinion in ChE · 36 · pp. 10082 Aaron Thole, Douglas D Frey, Govind Rao
  • Metal-semiconductor sorting of large-diameter single-wall carbon nanotubes by pH-dependent binding to a hydrophobic-interaction adsorbent 2021 Carbon · 175 · pp. 3082-3092 Payam Rezaei, Lisa Pfefferle, Douglas D Frey
  • Manufacturing Biological Medicines on Demand: Safety and Efficacy of GCSF in a Mouse Model of Total Body Irradiation 2020 Biotechnol. Prog. · 36 (3) · pp. e2970 Rajani Adija, Abhay Andar, David Bergenson, Sevda Deldari, Douglas D Frey, Xudong Ge, Yang Liu, Govind Rao, Lynn Wong
  • Evaluation of chromatofocusing as a capture method for monoclonal antibody products 2018 Journal of Chromatography A · 1568 · pp. 108-122 Douglas D Frey, Yang Liu, Sevda Deldari, Hui Guo, Chittoor Narahari, Ronald Bates, Ryan Swanson, Sanchayita Ghose, Zheng-Jian Li
  • Cell-Free Biomanufacturing 2018 Current Opinion Chemical Engineer · 22 · pp. 177-183 Brad C Bundy, J C Hunt, Micheal C Jewett, James R Swartz, David W Wood, Douglas D Frey, Govind Rao
  • Improving the recombinant human erythropoietin glycoslyation using microsome supplementation in CHO cell-free system 2018 Biotechnology and Bioengineering · 115 · pp. 1253-1264 Chandrasekhar Gurramkonda, Aniruddha Rao, Shayan Borhani, Manohar Pilli, Sevda Deldari, Xudong Ge, Douglas D Frey, Govind Rao
  • Cell free expression of a therapeutic protein: expression, purification, and characterization of recombinant streptokinase using a CHO lysate 2018 Biotechnology and Bioengineering · 118 · pp. 92-102 Douglas D Frey, Kevin Tran, Chandrasekhar Gurramkonda, Merrideth Cooper, Manohar Pilli, Nicholas Selock, T.-C. Han, Michael Tolosa, Adil Zuber, Chariz Penalber, N. Pezeshk, Iordan V Kostov, Leah Tolosa, David Wood, Govind Rao
  • Displacement chromatography of proteins using a retained pH front in a hydrophobic charge induction chromatography column 2015 J. Chromatogr. A · 1387 · pp. 53-59 Nuno Pinto, Douglas D Frey
  • Chromatofocusing 2015 Encyclopedia of Life Sciences · Encyclopedia of Life Sciences · 3 · pp. 38-45 Mayyada El-Sayed, Douglas D Frey
  • Development of Chromatofocusing Techniques Employing Mixed Mode Column Packings for Protein Separations 2014 J. Chromatogr. A · 1323 · pp. 57-65 Hui Guo, Douglas D Frey
  • High Performance Liquid Chromatography in the Undergraduate Chemical Engineering laboratory 2013 Chem. Eng. Ed. · 47 · pp. 15-24 Douglas D Frey, Hui Guo, Nikila Karnik
  • Monoclonal Antibody Heterogeneity Analysis and Deamidation Monitoring using High-Performance Cation-Exchange Chromatofocusing using Simple, Two Component Buffer Systems 2013 J. Chromatogr. A · 1283 · pp. 89-97 Xuezhen Kang, J.P. Klutzko, M. Hayes, Douglas D Frey

Creative Publications

  • Metal-semiconductor sorting of large-diameter single-wall carbon nanotubes by pH-dependent binding to a hydrophobic-interaction adsorbent 2021 Carbon · 175 · pp. 3082-3092 Payam Rezaei, Lisa Pfefferle, Douglas D Frey

Presentations

  • Application of Mechanistic Modeling for the Understanding of Tailing in High Performance Liquid Chromatography (HPLC). 2024 UMBC Graduate Experience, Achievements & Research Symposium (GEARS) Research Symposium · UMBC · Poster Douglas D Frey (Author), Govind Rao (Author), Revati Kadolkar (Author & Presenter)
  • Development of a novel analytical sensor utilizing Ni-NTA and His-tag affinity for at-line measurement of binding proteins. 2024 International Foundation for Process Analytical Chemistry (IFPAC) · International Foundation for Process Analytical Chemistry (IFPAC) · Poster Douglas D Frey (Author), Govind Rao (Author), Vikash Kumar (Author), Revati Kadolkar (Author & Presenter)
  • Application of mechanistic modeling for the understanding of tailing in HPLC 2023 IFPAC annual meeting · IFPAC · Poster Douglas D Frey (Author), Govind Rao (Author), Revati Kadolkar (Author & Presenter)
  • Applications of scientific machine learning to the modeling of chromatography 2022 PREP 2022: International conference on preparative and process chromatography · Poster Douglas D Frey, Aaron Thole, Revati Kadolkar, Pegah Rezaei
  • Chromatographic sorting of carbon nanotubes 2019 AIChE meeting · AIChE · Oral Presentation Douglas D Frey (Author), Payam Rezaei (Author & Presenter)
  • Design of protein purification methods for on-demand biologics 2019 PREP 2019 conference · PREP 2019 · Oral Presentation Douglas D Frey (Author), Sevda Deldari (Author & Presenter)
  • Monoclonal Antibody Capture Methods Using Chromatofocusing 2018 PREP 2018 · Oral Presentation Douglas D Frey (Author), Yang Liu (Author & Presenter)
  • Experimental and theoretical studies of protein adsorption onto ion exchange and mixed mode chromatographic column packings 2018 ACS (New Orleans) · American Chemical Society · Oral Presentation Douglas D Frey (Author & Presenter), Payam Rezaei (Author)
  • Development and validation of a purification platform for proteins expressed in a CHO cell-free system 2018 ACS (New Orleans) · American Chemical Society · Oral Presentation Douglas D Frey (Author), Sevda Deldari (Author & Presenter)
  • Evaluation of chromatofocusing and related ion exchange methods as alternatives to protein A chromatography in the purification of antibodies 2018 ACS (New Orleans) · American Chemical Society · Oral Presentation Douglas D Frey (Author), Yang Liu (Author & Presenter)
  • Investigation of charge-based chromatographic methods for sorting nanotube chiral forms 2018 ACS (New Orleans) · American Chemical Society · Oral Presentation Douglas D Frey (Author), Payam Rezaei (Author & Presenter)
  • Development and assessment of chromatofocusing and related alternatives to protein A chromatography for the purification of antibodies 2018 International Foundation Process Analytical Chemistry · Poster Douglas D Frey (Author), Yang Liu (Author & Presenter)
  • Prediction and correlation of protein adsorption in ion exchange and mixed mode chromatography 2018 International Foundation Process Analytical Chemistry · Poster Douglas D Frey (Author), Payam Rezaei (Author & Presenter)
  • Evaluation of chromatofocusing and related ion exchange methods as alternatives to protien A chromatography in the purification of therapeutic proteins 2017 International Conference on Preparative Chromatograhy · Poster Douglas D Frey (Author), Yang Liu (Author & Presenter)
  • Development and validation of an IMAC purification platform for His-tagged proteins expressed in a CHO cell-free system 2017 Regulatory Sciences for Biologics and Vaccines · Poster Douglas D Frey (Author), Sevda Deldari (Author & Presenter)
  • Fundamental studies of the mechanism of ion exchange chromatography 2017 Regulatory Sciences for Biologics and Vaccines · Poster Douglas D Frey (Author), Payam Rezaei (Author & Presenter)
  • Process development tools for the purification of therapeutic antibody products using non-affinity capture methods 2017 Regulatory Sciences for Biologics and Vaccines · Poster Douglas D Frey (Author), Yang Liu (Author & Presenter)
  • New methods for the chromatography of peptides and proteins 2014 Csaba Horvath Distinguished Lecture at Yale University · Department of Chemical Engineering, Yale University · Seminar
  • Novel chromatofocusing methods for elution of IgG from Protein A affinity columns 2014 2014 ACS National Meeting · ACS · Oral Presentation Nuno Pinto (Author & Presenter), Douglas D Frey (Author)
  • Hybrid methods for the chromatofocusing of proteins 2013 2013 ACS National Meeting · ACS · Oral Presentation Nuno Pinto (Author & Presenter), Douglas D Frey (Author)

Grants and Contracts

  • ATIP: Novel methods for determination of Hb A1c 2024 UMB/NIH · Grant Douglas D Frey (Principal), Kashif Munir (Co-Principal)
  • CASTing Biologics on Demand 2013 DARPA · Contract · Funded
  • Scalable Separation of Single Walled Carbon Nanotubes 2013 NSF,National Science Foundation 47.000 · Grant · Funded
  • Novel mixed-mode chromatographic processes NSF,National Science Foundation 47.000 · Grant · Funded
  • NSF Supplement: Scalable Separation of Single Walled Carbon Nanotubes NSF,National Science Foundation 47.000 · Grant · In-Progress

Patents and Intellectual Property

  • System and method for production of on-demand proteins in a portable unit for POC delivery 2025 Patent · No. US 12,221,603 B2 Govind Rao, Iordan V Kostov, Leah Tolosa, Xudong Ge, Douglas D Frey
  • System and method for production of on-demand proteins in a portable unit for POC delivery 2024 Patent · No. 16/660,993 Douglas D Frey, Govind Rao, Iordan V Kostov, Leah Tolosa, Xudong Ge
  • Factory on a chip for production of biologically derived medicine/biopharmaceuticals/biologics/biotherapeutics 2024 Patent · No. Application No. 18/329,643 Douglas D Frey, Govind Rao, Yordan Kostov, Abhay Andar, Sevda Deldari

Courses Taught

  • Separation Processes ENCH 445 · Fall 2020
  • Doctoral Diss Research ENCH 899 · Fall 2019
  • Transport I:Fluids ENCH 425 · Fall 2019
  • Transport I:Fluids ENCH 425 · Fall 2019
  • Doctoral Diss Research ENCH 899 · Spring 2019
  • Transport Phenomena ENCH 630 · Spring 2019
  • Separation Processes ENCH 445 · Fall 2016
  • Doctoral dissertation research ENCH 898 · Spring 2016
  • MS thesis research ENCH 799 · Spring 2016
  • Independent Research ENCH 648 · Spring 2016
  • Transport Phenomena ENCH 630 · Spring 2016